Facilities page icon showing normal colonic epithelial mucosa

PARKOS and NUSRAT LABORATORIES
Epithelial Pathobiology and Mucosal Inflammation Research Unit

Department of Pathology and Laboratory Medicine
Emory University School of Medicine, Atlanta, Georgia, USA

TECHNIQUES & EQUIPMENT

Core techniques and technologies in the laboratory include western blotting, immunofluorescence, immunohistochemistry, real-time PCR, colonic crypt isolation, mouse colonoscopy, tissue permeability measurements with Ussing chamber, automated gel filtration chromatography
, cloning/mutagenesis, RNAi and plasmid expression, viral vectors, in vitro cell culture models, in vivo imaging modalities, confocal microscopy and more!


CORE FACILITIES


Work in our laboratory is also supported by numerous research core facilities at Emory, some of which are even located within our own laboratory space. These facilities include the following (click on each facility name to find out more):

Image Analysis Core Facility (Director Asma Nusrat, MD; located within our lab)
Cell Culture and Monoclonal Antibody Core (Director Charles Parkos, MD, PhD; located within our lab)
Emory DNA Custom Cloning Core
Transgenic Mouse Core
Flow Cytometry Core
The Glycomics Center
WCI Pathology Core (tissue embedding, sectioning and staining)

In addition to the excellent resources and expertise here at Emory University, we also have on-going collaborations with several investigators at nearby at Georgia Institute of Technology (Georgia Tech).


Epithelial monolayer